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Can anyone help me understand how to pin down possible reasons for getting only 37% of my original assays vs. pulp duplicates data plotting within 10% when I generate a HARD plot? I have over 400 pairs of data from three different labs. At each of the 3 labs the reproducibility according this plot is between 30% and 40%. However when I generate a normal regression correlation plot my R2 is over 0.9 at all the Labs.
I imagined that my regression correlation graph would coincide with the HARD plot. Are these two graphs different?
How can I improve my HARD plot from 37% to 90% plotting within 10%? What is the implication of the 37%?